abstract：AIM:Adenosine monophosphate-activated protein kinase (AMPK), a vital regulator of glucose metabolism, may affect insulin secretion in beta-cells. However, the role of AMPK in beta-cell lipotoxicity remains unclear. Fenofibrate has been reported to regulate lipid homeostasis and is involved in insulin secretion in pancr...

journal_title：Acta pharmacologica Sinica

authors： Sun Y,Ren M,Gao GQ,Gong B,Xin W,Guo H,Zhang XJ,Gao L,Zhao JJ

更新日期：2008-04-01 00:00:00

abstract：AIM:To explore the anticancer effects and the molecular mechanisms of gambogic acid (GA) on Jurkat cells. METHODS:Cell viability was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Annexin V-fluorescein-isothiocyanate/propidium iodide, DNA defragmentation, and comet assay were used to d...

journal_title：Acta pharmacologica Sinica

authors： Wang Y,Chen Y,Chen Z,Wu Q,Ke WJ,Wu QL

更新日期：2008-03-01 00:00:00

abstract：AIM:Our previous study demonstrated an siRNA-mediated, allele-specific silencing of mutant genes that cause amyotrophic lateral sclerosis. To improve siRNA design for better therapeutic use of RNA interference, we systematically tested the base-pairing mismatch strategy in the design of asymmetric siRNA. METHODS:A nat...

journal_title：Acta pharmacologica Sinica

authors： Geng CM,Ding HL

更新日期：2008-02-01 00:00:00

abstract：AIM:The aim of the present study was to explore the mechanism for the Ca2+- dependent inactivation of the canonical transient receptor potential (TRPC) 7 channel expressed in human embryonic kidney 293 cells. METHOD:The whole-cell patch-clamp technique was used in the study. RESULTS:With Ca2+-free external solution, ...

journal_title：Acta pharmacologica Sinica

authors： Zhang H,Inoue R,Shi J,Jin XH,Li YQ

更新日期：2008-01-01 00:00:00

abstract：AIM:To characterize the in vitro bioactivities of rhodanine derivatives as novel peroxisome proliferator-activated receptor (PPAR) gamma modulators, based on a hit (SH00012671) identified during high-throughput screening (HTS) of a diverse synthetic compound library, and to preliminarily elucidate the structure-activit...

journal_title：Acta pharmacologica Sinica

authors： Liu Q,Zhang YY,Lu HL,Li QY,Zhou CH,Wang MW

更新日期：2007-12-01 00:00:00

abstract：AIM:HMGB1 (high-mobility group box-1) is a nuclear protein containing a consensus RB (retinoblastoma)-binding LXCXE motif. In this study, we studied the potential association of HMGB1 and RB and the in vitro and in vivo activities of HMGB1 in human breast cancer cells. METHODS:The protein-protein interaction was deter...

journal_title：Acta pharmacologica Sinica

authors： Jiao Y,Wang HC,Fan SJ

更新日期：2007-12-01 00:00:00

abstract：AIM:To investigate whether sodium ferulate (SF) can protect cortical neurons from glutamate-induced neurotoxicity and the mechanisms responsible for this protection. METHODS:Cultured cortical neurons were incubated with 50 micromol/L glutamate for either 30 min or 24 h, with or without pre-incubation with SF (100, 200...

journal_title：Acta pharmacologica Sinica

authors： Jin Y,Yan EZ,Fan Y,Guo XL,Zhao YJ,Zong ZH,Liu Z

更新日期：2007-12-01 00:00:00

abstract：AIM:To evaluate the effect of C-C chemokine receptor 3 (CCR3) blockade on pulmonary inflammation and mucus production in allergic mice. METHODS:We used the synthetic peptide of the CCR3 NH2-terminal as the immunizing antigen and generated murine monoclonal antibody against the human CCR3. In addition, the generated an...

journal_title：Acta pharmacologica Sinica

authors： Wang K,Shen HH,Li W,Huang HQ

更新日期：2007-11-01 00:00:00

abstract：:Hydrogen sulfide (H2S), which is well known traditionally as a toxic gas, has been proven to be produced endogenously by 3 enzymes in mammalian tissues and plays important roles in physiological and pathophysiological conditions. In the central nervous system, H2S functions as not only a neuromodulator, but also a neu...

journal_title：Acta pharmacologica Sinica

authors： Chen CQ,Xin H,Zhu YZ

更新日期：2007-11-01 00:00:00

abstract：AIM:To develop a robust, cell-based, high-content screening (HCS) assay based on receptor internalization for the identification of novel modulators of the epidermal growth factor receptor (EGFR). METHODS:Agonist-induced receptor internalization is part of the signaling cascade of EGFR. Fluorescent-tagged epidermal gr...

journal_title：Acta pharmacologica Sinica

authors： Wang J,Xie X

更新日期：2007-10-01 00:00:00

abstract：AIM:To investigate the effect of the Tob1 gene, a member of the Transducing Molecule of ErbB2/B-cell Translocation Ggene (TOB/BTG) family, by using the adenovirus-mediated expression of Tob1 on radiosensitivity in a human breast cancer cell line MDA-MB-231. METHODS:Cell survival was determined by clonogenic assay. Apo...

journal_title：Acta pharmacologica Sinica

authors： Jiao Y,Ge CM,Meng QH,Cao JP,Tong J,Fan SJ

更新日期：2007-10-01 00:00:00

abstract：AIM:In order to elucidate the molecular mechanism underlying the cardioprotection afforded by schisandrin B (Sch B), the effect of Sch B treatment on the sensitivity of mitochondria to Ca2+-stimulated permeability transition (PT) was investigated in rat hearts under normal and ischemia-reperfusion (I-R) conditions. RE...

journal_title：Acta pharmacologica Sinica

authors： Chiu PY,Leung HY,Siu AH,Poon MK,Ko KM

更新日期：2007-10-01 00:00:00

abstract：:In an effort to develop effective alternative strategies that increase the therapeutic efficacy and minimize the systemic toxicity of chemotherapeutic agents, more efforts are being directed towards the investigation of dietary supplements and other phytotherapeutic agents for their synergistic efficacy in combination...

journal_title：Acta pharmacologica Sinica

authors： Raina K,Agarwal R

更新日期：2007-09-01 00:00:00

abstract：:Androgen and androgen receptor (AR) signaling are crucial for the genesis of prostate cancer (PCa), which can often develop into androgen-ligand-independent diseases that are lethal to the patients. Recent studies show that even these hormone-refractory PCa require ligand-independent AR signaling for survival. As curr...

journal_title：Acta pharmacologica Sinica

authors： Lu J,Kim SH,Jiang C,Lee H,Guo J

更新日期：2007-09-01 00:00:00

abstract：AIM:To investigate the pharmacokinetic profile and tissue distribution of a novel phosphodiesterase type 5 inhibitor, 5-ethyl-2-{5-[4-(2-hydroxy-ethyl)-piperazine-1-sulfonyl]-2-propoxy-phenyl}-7-propyl-3,5-dihydro-pyrrolo(3,2-d)pyrimidin-4-one (SK-3530), in rats after administration of the (14)C-labeled compound. METH...

journal_title：Acta pharmacologica Sinica

authors： Yoo HH,Kim NS,Im GJ,Kim DH

更新日期：2007-08-01 00:00:00

abstract：AIM:To examine the protective effect of propofol in renal ischemia/reperfusion (I/R) injury and the role of heme oxygenase-1 (HO-1) in this process. METHODS:Sprague-Dawley rats were randomly divided into 3 groups: (i) sham-operated group; (ii) I/R group; and (iii) propofol group. Bilateral renal warm ischemia for 45 m...

journal_title：Acta pharmacologica Sinica

authors： Wang HH,Zhou HY,Chen CC,Zhang XL,Cheng G

更新日期：2007-08-01 00:00:00

abstract：AIM:To investigate the effect and mechanism of morphine on purine nucleotide catabolism. METHODS:The rat model of morphine dependence and withdrawal and rat C6 glioma cells in culture were used. Concentrations of uric acid in the plasma were measured by the uricase-rap method, adenosine deaminase (ADA) and xanthine ox...

journal_title：Acta pharmacologica Sinica

authors： Liu C,Liu JK,Kan MJ,Gao L,Fu HY,Zhou H,Hong M

更新日期：2007-08-01 00:00:00

abstract：AIM:To investigate the effects of small interfering RNA of cyclooxygenase-2 (COX-2) on the proliferation and apoptosis of human multiple myeloma RPMI8226 cells and its relation with the Bcl-2 family in vitro. METHODS:Transcription and expression of COX-2 in human myeloma RPMI8226 cells were checked by RT-PCR and Weste...

journal_title：Acta pharmacologica Sinica

authors： Li QB,Chen ZC,You Y,Zou P

更新日期：2007-07-01 00:00:00

abstract：AIM:To examine whether (-)-stepholidine (SPD) has a direct effect on the N-methyl- D-aspartic acid receptors (NMDAR) containing the NMDA receptor subunits NR2A or NR2B and to compare its effect with those of haloperidol (Hal) and clozapine (Cloz). METHODS:NMDAR was transiently expressed in human embryonic kidney 293 (...

journal_title：Acta pharmacologica Sinica

authors： Gu WH,Yang S,Shi WX,Zhen XC,Jin GZ

更新日期：2007-07-01 00:00:00

abstract：AIM:To investigate the immunoregulatory functions of water extracts of Hericium erinaceum (WEHE) focusing on natural killer (NK) cell-based anticancer activities. METHODS:Mouse splenocytes or purely isolated NK cells were stimulated with 1-100 mg/L WEHE for 24 h followed by co-culture with (51)Cr-labeled Yac-1 cells f...

journal_title：Acta pharmacologica Sinica

authors： Yim MH,Shin JW,Son JY,Oh SM,Han SH,Cho JH,Cho CK,Yoo HS,Lee YW,Son CG

更新日期：2007-06-01 00:00:00

abstract：AIM:To develop a high-throughput real-time assay based on molecular beacons to monitor the integrase 3'-processing reaction in vitro and apply it to inhibitor screening. METHODS:The recombinant human immunodeficiency virus (HIV)-1 integrase (IN) is incubated with a 38 mer oligonucleotide substrate, a sequence identica...

journal_title：Acta pharmacologica Sinica

authors： He HQ,Ma XH,Liu B,Zhang XY,Chen WZ,Wang CX,Cheng SH

更新日期：2007-06-01 00:00:00

abstract：AIM:The interaction of anthracycline anticancer drugs with chromatin, nucleosomes and histone H1 has been extensively studied. In the present study, for the first time, we have investigated the binding of anthracycline antibiotic, daunomycin, to free and cross-linked thymus core histones (CL-core) in solution and in th...

journal_title：Acta pharmacologica Sinica

authors： Rabbani A,Abdosamadi S,Sari-Saraf N

更新日期：2007-05-01 00:00:00

abstract：AIM:To compare high K(+ )-induced contraction and optimal resting tension measured by two commonly used techniques of hypertonic and isotonic K(+ ) in aortas with and without adventitial fat from various age rats. METHODS:Three age groups of rats (15, 25, and 62 weeks) were used to prepare thoracic aortic rings in whi...

journal_title：Acta pharmacologica Sinica

authors： Guan YF,Chen RH,Wang P,Qin Y,Su DF,Miao CY

更新日期：2007-05-01 00:00:00

abstract：AIM:To develop a novel, in situ gel system for nasal delivery of scopolamine hydrobromide (SCOP) and study its efficacy on motion sickness. METHODS:SCOP in situ gels at 0.2%, 0.5%, and 1.0% gellan gum concentration (w/v) were prepared, respectively, and characterized in terms of viscosity, in vitro release, and nasal ...

journal_title：Acta pharmacologica Sinica

authors： Cao SL,Zhang QZ,Jiang XG

更新日期：2007-04-01 00:00:00

abstract：AIM:To examine the role of atorvastatin on volume-overload-induced heart failure and to test the hypothesis that atorvastatin inhibits MMP-2 and 9 expression in heart failure with non-ischemic etiology. METHODS:Arteriovenous (AV) fistula-treated rats were administered with atorvastatin (3 mg/kg/d) or vehicle for 17 we...

journal_title：Acta pharmacologica Sinica

authors： Cheng G,Xu G,Cai HW,Wang HH,Bao XF

更新日期：2007-04-01 00:00:00

abstract：AIM:To study the effect of gum mastic, a natural resin, on the proliferation of androgen-independent prostate cancer PC-3 cells, and further investigate the mechanisms involved in this regulatory system, taking nuclear factor kappaB (NF-kappaB) signal as the target. METHODS:3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltet...

journal_title：Acta pharmacologica Sinica

authors： He ML,Li A,Xu CS,Wang SL,Zhang MJ,Gu H,Yang YQ,Tao HH

更新日期：2007-03-01 00:00:00

abstract：AIM:To simultaneously investigate the contribution of the alpha 1 chain of collagen type 1 (COL1A1) and alpha2-HS-glycoprotein (AHSG) genes to the variation of bone geometric parameters in both Caucasians and Chinese. METHODS:Six hundred and five Caucasian individuals from 157 nuclear families and 1228 Chinese subject...

journal_title：Acta pharmacologica Sinica

authors： Jiang H,Lei SF,Xiao SM,Chen Y,Sun X,Yang F,Li LM,Wu S,Deng HW

更新日期：2007-03-01 00:00:00

abstract：AIM:To evaluate the pharmacokinetic characteristics of L-valyl-ara-C, a peptidomimetic prodrug of ara-C. METHODS:After the synthesis of L-valyl-ara-C, the in vitro stability of L-valyl-ara-C was examined in various biological media. Plasma pharmacokinetic profiles of ara-C and L-valyl-ara-C were also evaluated in rats...

journal_title：Acta pharmacologica Sinica

authors： Cheon EP,Han HK

更新日期：2007-02-01 00:00:00

abstract：AIM:G protein-coupled inwardly rectifying potassium channels (GIRK) are important for neuronal signaling and membrane excitability. In the present study, we intend to find whether GIRK channels express functionally in adult rat dorsal root ganglion (DRG) neurons. METHODS:We used RT-PCR to detect mRNA for 4 subunits of...

journal_title：Acta pharmacologica Sinica

authors： Gao XF,Zhang HL,You ZD,Lu CL,He C

更新日期：2007-02-01 00:00:00

abstract：AIM:To develop a simple method for monitoring protein localization of epidermal growth factor (EGF) in living cells. METHODS:Enhanced green fluorescent protein (EGFP) was used as an autofluorescent tag to label EGF ligands. SDS-PAGE and Western blot analysis were used to detect the expression of the EGFP-tagged EGF (E...

journal_title：Acta pharmacologica Sinica

authors： Jiang H,Zhang J,Shi BZ,Xu YH,Li ZH,Gu JR

更新日期：2007-01-01 00:00:00

abstract：AIM:To study whether urotensin II (UII), a potent vasoconstrictive peptide, is involved in the development of cardiac hypertrophy and fibrogenesis of rats induced by isoproterenol (ISO). METHODS:Thirty male Wistar rats were randomly divided into 3 groups. Group 1 was the healthy control group, group 2 was the ISO grou...

journal_title：Acta pharmacologica Sinica

authors： Zhang YG,Li YG,Liu BG,Wei RH,Wang DM,Tan XR,Bu DF,Pang YZ,Tang CS

更新日期：2007-01-01 00:00:00

abstract：AIM:It is generally assumed that only unbound drugs can reach the site of action by diffusing across the membranes and exerting pharmacological effects by interacting with receptors. Recent research has shown that the percentage of free drugs may depend on the total drug concentration. The aim of the paper is to verify...

journal_title：Acta pharmacologica Sinica

authors： Kalitynski R,Dawidowicz AL,Poszytek J

更新日期：2006-12-01 00:00:00

abstract：AIM:The role of caveolin-1 (Cav-1) in angiogenesis remains poorly understood. The endothelial nitric oxide (NO) synthase (eNOS), a caveolin-interacting protein, was demonstrated to play a predominant role in vascular endothelial growth factor (VEGF) -induced angiogenesis. The purpose of our study was to examine the rol...

journal_title：Acta pharmacologica Sinica

authors： Pan YM,Yao YZ,Zhu ZH,Sun XT,Qiu YD,Ding YT

更新日期：2006-12-01 00:00:00

abstract：AIM:To examine the in vitro antitumor activity of resveratrol against multiple myeloma (MM) cell lines (RPMI 8226, U266, and KM3), and the mechanisms involved. METHODS:The growth inhibition of resveratrol was determined by 3-(4, 5-dimethyl-2-thiazyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The effect of resve...

journal_title：Acta pharmacologica Sinica

authors： Sun CY,Hu Y,Guo T,Wang HF,Zhang XP,He WJ,Tan H

更新日期：2006-11-01 00:00:00

abstract：AIM:To investigate the effects of deferiprone on doxorubicin-induced cardiotoxicity and determine its protection on cardiac contractility in vivo at tissue level. METHODS:Spontaneously-beating isolated atria from rats were pretreated with deferiprone for 10 min at 1.2 mmol/L or 0.3 mmol/L, respectively before co-incub...

journal_title：Acta pharmacologica Sinica

authors： Xu LJ,Jin L,Pan H,Zhang AZ,Wei G,Li PP,Lu WY

更新日期：2006-10-01 00:00:00

abstract：AIM:To examine the effect of curcumin on oxidative DNA damage and cell apoptosis and injury caused by the reaction of methylglyoxal(MG) with amino acids. METHODS:We used DNA strand breaks to examine the effect of curcumin on oxidative DNA damage. In addition, reactive oxygen species(ROS) formation occurs in MG-treated...

journal_title：Acta pharmacologica Sinica

authors： Chan WH,Wu HJ

更新日期：2006-09-01 00:00:00

abstract：AIM:(-)Stepholidine (SPD) is an active ingredient of the Chinese herb Stephania intermedia, which binds to the dopamine D(1) and D(2) like receptors. Biochemical, electrophysiological and behavioural experiments have provided strong evidence that SPD is both a D(1) and a D(2) antagonist, which could make SPD a unique a...

journal_title：Acta pharmacologica Sinica

authors： Ellenbroek BA,Zhang XX,Jin GZ

更新日期：2006-09-01 00:00:00

abstract：AIM:To investigate whether IL-12p40 plays a crucial role in regulating islet allograft rejection in a streptozotocin (STZ)-induced diabetes mouse model. METHODS:C57BL/6 and IL-12p40 gene knockout mice were selected as recipient mice, to which the diabetes was induced with a treatment of STZ (150-200 mg/kg) by a single...

journal_title：Acta pharmacologica Sinica

authors： Bi EG,Shi W,Zou J,Hao ZH,Li ZH,Cai D,Zhang HQ,Sun B

更新日期：2006-08-01 00:00:00

abstract：AIM:To investigate the proliferation of vascular smooth muscle cells (VSMC) affected by ginsenoside Rg1 and further explore the molecular mechanism of ginsenoside Rg1 using proteomics. METHODS:The proliferation of VSMC was measured by MTS assay kit and flow cytometry. Proteomic alterations were analyzed using two-dime...

journal_title：Acta pharmacologica Sinica

authors： Ma ZC,Gao Y,Wang YG,Tan HL,Xiao CR,Wang SQ

更新日期：2006-08-01 00:00:00

abstract：AIM:Our previous study indicated that there are two types of Ca2+ release events seen in intact mouse bladder tissue. In this study our aim is to investigate the mechanism that underlies the phenomena of Ca2+ release in smooth muscle. METHODS:Single cells were isolated and tissue segments were prepared by cutting the ...

journal_title：Acta pharmacologica Sinica

authors： Wang M,Chen Z,Xing Y,Zhang X,Dong XZ,Ji GJ

更新日期：2006-07-01 00:00:00